Signalling

Part:BBa_I756034

Designed by: Jennifer Lei   Group: iGEM07_Bay_Area_RSI   (2007-10-25)


CD18-gsl-Nub

CD18-gsl-Nub

CD18 is the second half of the LFA1 signaling dimer which binds to ICAM. To induce an output from ligand mediated signaling, we used the split ubiquitin system. We designed LFA1 dimerisation to produce the same output as our other signaling receptors - mLexA-VP16.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 904
    Illegal XbaI site found at 2497
    Illegal PstI site found at 1167
    Illegal PstI site found at 1419
    Illegal PstI site found at 1938
    Illegal PstI site found at 1960
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 904
    Illegal PstI site found at 1167
    Illegal PstI site found at 1419
    Illegal PstI site found at 1938
    Illegal PstI site found at 1960
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 904
    Illegal BamHI site found at 177
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 904
    Illegal XbaI site found at 2497
    Illegal PstI site found at 1167
    Illegal PstI site found at 1419
    Illegal PstI site found at 1938
    Illegal PstI site found at 1960
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 904
    Illegal XbaI site found at 2497
    Illegal PstI site found at 1167
    Illegal PstI site found at 1419
    Illegal PstI site found at 1938
    Illegal PstI site found at 1960
    Illegal AgeI site found at 2386
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1373
    Illegal SapI site found at 2321


[edit]
Categories
Parameters
None