Signalling

Part:BBa_I756033

Designed by: Jennifer Lei   Group: iGEM07_Bay_Area_RSI   (2007-10-25)


CD11a

LFA1 is a human transmembrane receptor composed of CD18 and CD11a which bind to ICAM. In acute myocardial infarction ICAM is exposed on the cell surface and therefore is one of the ideal targets for repairing damaged heart tissue.

To transmit the signal, we used a split ubiquitin system (Cub and NubG) that will reconstitute mLexA-VP16 in vivo. MLexA-VP16 translocates to nucleus where is functions as the switch to upregulate expression of the rescue circuit, death circuit and cell adhesion circuit via the binding to the lexA operator minimal promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1742
    Illegal XbaI site found at 3532
    Illegal PstI site found at 160
    Illegal PstI site found at 418
    Illegal PstI site found at 433
    Illegal PstI site found at 991
    Illegal PstI site found at 1162
    Illegal PstI site found at 1540
    Illegal PstI site found at 2035
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1742
    Illegal PstI site found at 160
    Illegal PstI site found at 418
    Illegal PstI site found at 433
    Illegal PstI site found at 991
    Illegal PstI site found at 1162
    Illegal PstI site found at 1540
    Illegal PstI site found at 2035
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1742
    Illegal BglII site found at 975
    Illegal BglII site found at 1001
    Illegal BamHI site found at 2879
    Illegal BamHI site found at 3462
    Illegal XhoI site found at 81
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1742
    Illegal XbaI site found at 3532
    Illegal PstI site found at 160
    Illegal PstI site found at 418
    Illegal PstI site found at 433
    Illegal PstI site found at 991
    Illegal PstI site found at 1162
    Illegal PstI site found at 1540
    Illegal PstI site found at 2035
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1742
    Illegal XbaI site found at 3532
    Illegal PstI site found at 160
    Illegal PstI site found at 418
    Illegal PstI site found at 433
    Illegal PstI site found at 991
    Illegal PstI site found at 1162
    Illegal PstI site found at 1540
    Illegal PstI site found at 2035
    Illegal NgoMIV site found at 75
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 901
    Illegal BsaI.rc site found at 2274


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