Composite

Part:BBa_I756031

Designed by: Omar Khan   Group: iGEM07_Bay_Area_RSI   (2007-10-25)


mLexA-dnaEn-p85alpha

p85alpha is a subunit of PI-3K. It is linked, at its N terminus with mLexA-dnaEn. This construct was cloned into pcDNA3.1(+)Zeo with the sites: XbaI and EcoRV.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 607
    Illegal XbaI site found at 3258
    Illegal PstI site found at 651
    Illegal PstI site found at 1555
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 607
    Illegal PstI site found at 651
    Illegal PstI site found at 1555
    Illegal NotI site found at 633
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 607
    Illegal BglII site found at 537
    Illegal BamHI site found at 617
    Illegal XhoI site found at 640
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 607
    Illegal XbaI site found at 3258
    Illegal PstI site found at 651
    Illegal PstI site found at 1555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 607
    Illegal XbaI site found at 3258
    Illegal PstI site found at 651
    Illegal PstI site found at 1555
    Illegal NgoMIV site found at 1076
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2812


[edit]
Categories
Parameters
None