Composite

Part:BBa_I756029

Designed by: Omar Khan   Group: iGEM07_Bay_Area_RSI   (2007-10-25)


Syk-dnaEc-VP16AD

Syk kinase with SH2 domains coupled at the C terminus to dnaEc-VP16(activation domain). This construct was inserted into pcDNA3.1(+)Zeo using XbaI and EcoRV.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2163
    Illegal PstI site found at 1360
    Illegal PstI site found at 1763
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1979
    Illegal PstI site found at 1360
    Illegal PstI site found at 1763
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1461
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2163
    Illegal PstI site found at 1360
    Illegal PstI site found at 1763
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2163
    Illegal PstI site found at 1360
    Illegal PstI site found at 1763
    Illegal NgoMIV site found at 1892
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None