Reporter

Part:BBa_I756027

Designed by: Jennifer Lei   Group: iGEM07_Bay_Area_RSI   (2007-10-25)


LexAopMLP-GFP-SV40PA

Reporter construct for signaling circuit. GFP under the control of the lexAopMLP promoter and a SV40 polyA tail. SCFV-Epor-gp130, FcGamma, Syk, P85, CD11a, CD18-Cub, and CD18-nubG all have output of lexA-VP16 which will bind the LexAop and turn on the expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 988
    Illegal PstI site found at 994
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 994
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 961
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 988
    Illegal PstI site found at 994
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 988
    Illegal PstI site found at 994
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None