Coding

Part:BBa_I756004

Designed by: Jennifer Lei   Group: iGEM07_Bay_Area_RSI   (2007-08-08)


IgM signal seq - Vh-linker-VL

R1 - Xba1- Nde1 - kozak - mouse igm signal sequence - mouse myosin heavy Vh - gsg - mouse myosin heavy VL - kpn1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5
    Illegal XbaI site found at 11
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
    Illegal BamHI site found at 678
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5
    Illegal XbaI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5
    Illegal XbaI site found at 11
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 507


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Categories
Parameters
None