Composite

Part:BBa_I751102

Designed by: Kenichiro Iwasaki   Group: iGEM07_Tokyo_Tech   (2007-10-23)

J54140dP + hybrid promoter (lux-cI)

This part is made for B-side part in our project.

This part has a hybrid promoter, plux-cI. This promoter accepts two inputs. One is repression of cI repressor, and the other is activation of AHL.

GFP is assigned in the downstream of this hybrid promoter.

This part is made in case BBa_I751101 (which has plux-lac) doesn't work.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1200
    Illegal BamHI site found at 1116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1892


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Parameters
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