Inverter

Part:BBa_I750006

Designed by: Patricia Illing   Group: iGEM07_Melbourne   (2007-07-02)


OmpR inverter

This part inverts the activating output of OmpR so that transcription of a desired coding sequence can be triggered in the response to reduced EnvZ activity. The endogenous protein OmpR is phosphorylated by EnvZ. Phosphorylated OmpR then binds its response element (BBa_R0084) activating transcription. By placing the cI inverter system (BBa_Q04510) behind this promotor transcription of a desired product can be triggered by reduced EnvZ activity. This part is intended to invert the activating output of the red light receptor system BBa_M30109 in the absense of light to a repression of transcription. As a result transcription should be activated in the presence of red light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None