Composite

Part:BBa_I750004

Designed by: Phillip Dodson   Group: iGEM07_Melbourne   (2007-06-30)


red light receptor GFP test harness

BBa_M30109 used as the receptor to phosphorylate endogenous OmpR OmpR then binds to OmpC promotor (BBa_R0084) to drive GFP expression using Pops GFP converter BBa_E0241.

BBa_E0241 Ribosome binding site : BBa_B0032

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 5204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1702
    Illegal BglII site found at 4190
    Illegal BamHI site found at 65
    Illegal XhoI site found at 2297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1255
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5120


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Categories
Parameters
None