Coding

Part:BBa_I746919

Designed by: Stefan Milde   Group: iGEM07_Cambridge   (2008-09-30)

superfolder GFP with 6-his tag

Coding region of superfolder GFP (see part description of I746916 for source and other information about superfolder GFP) with C-terminal 6-his tag for assembly into constructs that allow 6-his purification of this GFP variant for in vitro analysis.

More information about the properties of this GFP in relation to the currently used mut3GFP can be found at: http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


[edit]
Categories
Parameters
None