Generator

Part:BBa_I746903

Designed by: Stefan Milde   Group: iGEM07_Cambridge   (2008-10-01)


T7 promoter driving 6-his tagged mut3 GFP

This part is used for purification of mut3 GFP via its 6-his tag. It is driven by the T7 promoter. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of the tagged mut3 GFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693


[edit]
Categories
Parameters
None