Generator

Part:BBa_I746902:Design

Designed by: Stefan Milde   Group: iGEM07_Cambridge   (2008-10-01)


pbad promoter driving 6-his tagged mut3 GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1880
    Illegal SapI site found at 961


Design Notes

no special considerations.


Source

The part was assembled from intermediates via standard assembly.

References