Intermediate

Part:BBa_I746309

Designed by: Stefan Milde   Group: iGEM07_Cambridge   (2007-09-12)


phiR73 delta under control of pBAD/araC promoter

This is a construction intermediate. It consists of the arabinose-induced pBAD/araC promoter and the phiR73 delta activator. The phiR73 delta does contain a RBS (B0034)!


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None