Part:BBa_I746220
AIP Detector
This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.
In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick detects group I AIP.
The device incorporates the sensing infrastructure described here with the permeability device here so that the signal peptide is able to cross the outer membrane of E. coli to be detected.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 3105
Illegal NheI site found at 3128 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 426
Illegal BamHI site found at 1310 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3397
Illegal AgeI site found at 3763
Illegal AgeI site found at 4390
Illegal AgeI site found at 4908 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2877
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