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Intermediate

Part:BBa_I746204

Designed by: Narin Hengrung   Group: iGEM07_Cambridge   (2007-08-31)


FepA L8 Mutant with Elowitz RBS and Standard Terminator

This is an intermediate on the way to the construction of a permeability device for the outer membrane of E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 2240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal SpeI site found at 2241
    Illegal PstI site found at 2255
    Illegal NotI site found at 27
    Illegal NotI site found at 2248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 2241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal XbaI site found at 36
    Illegal SpeI site found at 2241
    Illegal PstI site found at 2255
    Illegal AgeI site found at 278
    Illegal AgeI site found at 644
    Illegal AgeI site found at 1271
    Illegal AgeI site found at 1780
  • 1000
    COMPATIBLE WITH RFC[1000]


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