Intermediate

Part:BBa_I746203

Designed by: Narin Hengrung   Group: iGEM07_Cambridge   (2007-10-21)


Intermediate for Outer Membrane Permeability Device

This part contains the coding region for the L8T mutant of FepA. When expressed, this protein forms an open diffusion channel in the outer membrane of E. coli, allowing molecules of at least 1400Da to pass through. I746201 and I746202 both are devices to express this protein at different levels. This part does not have a promotor, to allow new constructs to easily be made.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 256
    Illegal AgeI site found at 622
    Illegal AgeI site found at 1249
    Illegal AgeI site found at 1767
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None