Part:BBa_I746101

I746100 (AIP sensor infrastructure - agrC + agrA with RBSes) + B0015 (Terminator)

This part is simply the AIP sensor infrastructure (I746100) from the agr system with a standard registry terminator (B0015) downstream.

The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.

In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.

This part contains the coding regions for group I agrC and agrA (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a BamHI site.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 383
  Illegal BamHI site found at 1267
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]