Plasmid

Part:BBa_I745012

Designed by: Princeton iGEM2007   Group: iGEM07_Princeton   (2007-10-26)


pLV-TH-siGata3-3'

This plasmid produces siGata3. In the presence of aTc, (in a TetR cell line), more RNAi is produced.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5392
    Illegal XbaI site found at 5884
    Illegal SpeI site found at 4329
    Illegal PstI site found at 2433
    Illegal PstI site found at 2938
    Illegal PstI site found at 5386
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5392
    Illegal NheI site found at 4298
    Illegal NheI site found at 4302
    Illegal NheI site found at 6143
    Illegal SpeI site found at 4329
    Illegal PstI site found at 2433
    Illegal PstI site found at 2938
    Illegal PstI site found at 5386
    Illegal NotI site found at 1144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5392
    Illegal BglII site found at 474
    Illegal BglII site found at 2687
    Illegal BglII site found at 4280
    Illegal BglII site found at 4985
    Illegal BglII site found at 5719
    Illegal BglII site found at 9131
    Illegal BamHI site found at 5077
    Illegal XhoI site found at 3086
    Illegal XhoI site found at 3482
    Illegal XhoI site found at 5085
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5392
    Illegal XbaI site found at 5884
    Illegal SpeI site found at 4329
    Illegal PstI site found at 2433
    Illegal PstI site found at 2938
    Illegal PstI site found at 5386
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5392
    Illegal XbaI site found at 5884
    Illegal SpeI site found at 4329
    Illegal PstI site found at 2433
    Illegal PstI site found at 2938
    Illegal PstI site found at 5386
    Illegal NgoMIV site found at 2821
    Illegal NgoMIV site found at 4855
    Illegal NgoMIV site found at 6176
    Illegal AgeI site found at 2200
    Illegal AgeI site found at 3541
    Illegal AgeI site found at 9485
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 456
    Illegal BsaI site found at 5701
    Illegal BsaI site found at 7531
    Illegal BsaI.rc site found at 5605
    Illegal SapI.rc site found at 1108
    Illegal SapI.rc site found at 8613


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Categories
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