Part:BBa_I745001

pFUGW

This plasmid allows constitutive expression of EGFP.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2012
  Illegal XbaI site found at 1248
  Illegal XbaI site found at 2006
  Illegal SpeI site found at 1175
  Illegal SpeI site found at 7329
  Illegal SpeI site found at 8003
  Illegal PstI site found at 2629
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2012
  Illegal SpeI site found at 1175
  Illegal SpeI site found at 7329
  Illegal SpeI site found at 8003
  Illegal PstI site found at 2629
  Illegal NotI site found at 1998
  Illegal NotI site found at 8039
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2012
  Illegal BglII site found at 2793
  Illegal BglII site found at 2859
  Illegal BglII site found at 2900
  Illegal BglII site found at 7092
  Illegal BglII site found at 8091
  Illegal BamHI site found at 1254
  Illegal XhoI site found at 1236
  Illegal XhoI site found at 8046
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2012
  Illegal XbaI site found at 1248
  Illegal XbaI site found at 2006
  Illegal SpeI site found at 1175
  Illegal SpeI site found at 7329
  Illegal SpeI site found at 8003
  Illegal PstI site found at 2629
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2012
  Illegal XbaI site found at 1248
  Illegal XbaI site found at 2006
  Illegal SpeI site found at 1175
  Illegal SpeI site found at 7329
  Illegal SpeI site found at 8003
  Illegal PstI site found at 2629
  Illegal NgoMIV site found at 3488
  Illegal NgoMIV site found at 4521
  Illegal NgoMIV site found at 4582
  Illegal NgoMIV site found at 4696
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2882
  Illegal BsaI site found at 8073
  Illegal BsaI.rc site found at 487
  Illegal BsaI.rc site found at 2642
  Illegal BsaI.rc site found at 6224
  Illegal BsaI.rc site found at 7962
  Illegal SapI site found at 5141