Plasmid

Part:BBa_I745000

Designed by: Weiss Lab   Group: iGEM07_Princeton   (2007-10-26)


Backbone TRE plasmid

This backbone consists of the TRE promoter, activated when the reverse transactivator, rtTA, and Doxycycline together bind the promoter. An IRES (internal ribosomal binding site) follows the TRE promoter and EGFP follows the IRES.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1488
    Illegal XbaI site found at 8889
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1488
    Illegal NheI site found at 1507
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal NotI site found at 85
    Illegal NotI site found at 2865
    Illegal NotI site found at 8876
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1488
    Illegal BglII site found at 3630
    Illegal BglII site found at 3696
    Illegal BglII site found at 3737
    Illegal BglII site found at 7929
    Illegal BglII site found at 8934
    Illegal BamHI site found at 1546
    Illegal XhoI site found at 8883
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1488
    Illegal XbaI site found at 8889
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1488
    Illegal XbaI site found at 8889
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal NgoMIV site found at 4325
    Illegal NgoMIV site found at 5358
    Illegal NgoMIV site found at 5419
    Illegal NgoMIV site found at 5533
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3719
    Illegal BsaI site found at 8916
    Illegal BsaI.rc site found at 3479
    Illegal BsaI.rc site found at 7061
    Illegal BsaI.rc site found at 8799
    Illegal SapI site found at 5978
    Illegal SapI.rc site found at 49


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