Coding
sam5

Part:BBa_I742141

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-23)

sam5 (coumarate hydroxylase) coding sequence

This is the coding sequence of sam5, encoding 4-coumarate-3-hydroxylase from the bacterium Saccharothrix espanaensis. The enzyme converts p-coumaric acid to caffeic acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006). Note: contains a PstI site. This will be removed by site directed mutagenesis in due course but is still present, near the 3' end, in this version.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1420
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1420
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1420
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1420
    Illegal NgoMIV site found at 21
    Illegal NgoMIV site found at 29
    Illegal NgoMIV site found at 779
    Illegal NgoMIV site found at 952
    Illegal NgoMIV site found at 1354
    Illegal NgoMIV site found at 1358
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 553


[edit]
Categories
//function/biosynthesis
Parameters
None