Composite

Part:BBa_I742138

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-22)


B-carotene (organge pigment) producing operon without PstI sites

B-carotene is an intermeidate in the Zeaxanthin biosynthesis pathway.

The four genes, CrtE, CrtB, CrtI and CrtY, encode four enzymes which sequentially convert farnesyl diphosphate (produced by the mevlonate pathway) into B-carotene.

More information on this biosynthesis pathway may be found on the [http://2007.igem.org/Edinburgh/Yoghurt/Design#Colour_Production Edinburgh Yoghurt Wiki]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1055
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3023
    Illegal NgoMIV site found at 3153
    Illegal AgeI site found at 757
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None