Coding

Part:BBa_I742133

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-22)


CrtIB genes with PstI site mutated out

CrtIB encodes two enzymes, which make up steps three and two in the carotenoid biosynthesis pathway respectively.

CrtI encodes phytoene desaturase and CrtB encodes Phytoene synthase.

Both cloned genes still have their native ribosome binding sites.

More information about the carotenoid synthesis pathway may be found on the [http://2007.igem.org/Edinburgh/Yoghurt/Design#Colour_Production Edinburgh Yoghurt Wiki]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2061
    Illegal NgoMIV site found at 2191
  • 1000
    COMPATIBLE WITH RFC[1000]


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