Part:BBa_I742132

Division-PoPper: Cell division --> PoPS

This device gives PoPS-output on cell division. The DNA flanked by the opposing repeats of the dif-site is inverted and allows transcription of both any downstream element and a repressor which, upon sufficient build-up, switches off the promoter which controls downstream transcription. Inversion only occurs when the XerCD complex is activated, and this only occurs at cell division.

Therefore this device produces a short burst of PoPS as a function of cell division. Any added downstream elements will likewise be activated, briefly, as a function of cell division.

This construct was ordered from GeneArt but at the time of writing, synthesis has not been successful, probably due to the inverse repeat nature of some of the elements. If synthesis is ultimately successful, we will deposit this construct in the Registry.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]