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Part:BBa_I742127

Designed by: Caroline Dahl   Group: iGEM07_Edinburgh   (2007-10-22)


dif-site + reverse Plac

The dif-site is a site for recombination at bacterial replication. When combined with a downstream direct repeat dif-site, flanked sequence will excise (including the reverse Plac). When combined with a reverse dif-site downstream, flanked DNA will invert.

Please note that the above hypothetical workings needs be tested for frequency and occurrance in vivo.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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