Coding

Part:BBa_I742119

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-19)


crtIB with both native ribosome binding sites

phytoene dehydrogenase and phytoene synthase from Pantoea ananatis

phytoene dehydrogenase produced Lycopene from phytoene

Lycopene is a red pigment with antioxident properties

Phytoene synthase produced phytoene from geranylgeranyl diphosphate

Both enzymes are part of the carotenoid biosynthesis pathway

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
    Illegal NgoMIV site found at 2061
    Illegal NgoMIV site found at 2191
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None