Coding

Part:BBa_I742112

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-19)

enoyl CoA hydratase (ech) gene

The sequence of enoyl CoA hydratase (ech) was derived from the Pseudomonas fluorescens genome. The sequence was submitted to GeneArt for synthesis including codon optimisation (Pseudomonas has a very high GC content whereas E. coli and Lactobacillus have much lower GC content).

Ech converts feruloyl CoA to vanillin (fig. 3) and catalyses the final stage of the vanillin biosynthesis pathway.

Involved in the synthesis of 'natural' vanilla flavouring.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]


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