Coding

Part:BBa_I741107

Designed by: Garrett Tobin   Group: iGEM07_Penn_State   (2007-06-25)


xylA and xylB metabolizing proteins plus region inbetween (with silent mutations)

This part makes xylA and xylB metabolizing protein. It also includes the DNA region between the two open reading frames. xylA is a subunit of xylose isomerase. xylA catalyzes the reaction D-xylose = D-xylulose. xylB is a xylulokinase. xylB catalyzes the reactions D-xylulose + ATP -> D-xylulose-5-phosphate + ADP, and ATP + 1-deoxy-D-xylulose -> 1-deoxy-D-xylulose 5-phosphate + ADP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1409
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1614
    Illegal AgeI site found at 997
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 115


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Parameters
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