Coding

Part:BBa_I741106

Designed by: Garrett Tobin   Group: iGEM07_Penn_State   (2007-06-24)


xylA and xylB metabolizing proteins plus region inbetween

This part makes xylA and xylB metabolizing protein. It also includes the DNA region between the two open reading frames. xylA is a subunit of xylose isomerase. xylA catalyzes the reaction D-xylose = D-xylulose. xylB is a xylulokinase. xylB catalyzes the reactions D-xylulose + ATP -> D-xylulose-5-phosphate + ADP, and ATP + 1-deoxy-D-xylulose -> 1-deoxy-D-xylulose 5-phosphate + ADP

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1587
    Illegal PstI site found at 1959
    Illegal PstI site found at 2338
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1587
    Illegal PstI site found at 1959
    Illegal PstI site found at 2338
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1409
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1587
    Illegal PstI site found at 1959
    Illegal PstI site found at 2338
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1587
    Illegal PstI site found at 1959
    Illegal PstI site found at 2338
    Illegal NgoMIV site found at 1614
    Illegal AgeI site found at 997
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 115


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Categories
Parameters
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