Composite

Part:BBa_I739013

Designed by: Stefan Luzi   Group: iGEM07_ETHZ   (2007-10-19)

Composite of BBa_I739001, BBa_I739002 and BBa_I739003

Part Structure

This composite Biobrick consists of part BBa_I739001, part BBa_I739002 and part BBa_I739003.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. The composite is cloned into plasmid pBR322BB1.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 898
    Illegal NheI site found at 921
    Illegal NheI site found at 2257
    Illegal NheI site found at 2280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None