Reporter

Part:BBa_I737008

Designed by: Patrick King   Group: iGEM07_Calgary   (2007-07-12)


OmpF promoter controlling GFP coding region.

This is the OmpF promoter (R0084) ligated to a GFP reporter (I13504).

In typical e.coli cells, the activity of Omp promoters varies with media osmolarity, and baseline expression is observed in these cells. However, when transformed in an EnvZ knockout strain (such as CP919, part number BBa_V1012) there should be little or no expression.

This part was designed to serve as a test reporter for part M30109, the light sensor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 778


[edit]
Categories
Parameters
None