Plasmid

Part:BBa_I735007

Designed by: Giovanni Russo   Group: iGEM07_Naples   (2007-09-28)


Cloning CDS PHO80 mutated in EX-Ptet-S-rbsRFP-P "RFP reporter"

We cloned PHO80CDS mutated in EX-Ptet-S-rbsRFP-P "RFP reporter" plasmid

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3817
    Illegal XbaI site found at 3832
    Illegal SpeI site found at 892
    Illegal PstI site found at 1775
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3817
    Illegal SpeI site found at 892
    Illegal PstI site found at 1775
    Illegal NotI site found at 1768
    Illegal NotI site found at 3823
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3817
    Illegal XhoI site found at 90
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3817
    Illegal XbaI site found at 3832
    Illegal SpeI site found at 892
    Illegal PstI site found at 1775
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3817
    Illegal XbaI site found at 3832
    Illegal SpeI site found at 892
    Illegal PstI site found at 1775
    Illegal AgeI site found at 1471
    Illegal AgeI site found at 1583
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 590
    Illegal BsaI.rc site found at 2856


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