Part:BBa_I732961

Ptemplate1 + RBS.ARL4A0604 + RBS.LRLa.TERM

• RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.

The original part is now BBa_I732952-I732964.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 2346
  Illegal EcoRI site found at 4481
  Illegal XbaI site found at 4646
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4481
  Illegal SpeI site found at 2347
  Illegal PstI site found at 2361
  Illegal NotI site found at 2354
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4481
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 2347
  Illegal EcoRI site found at 4481
  Illegal XbaI site found at 4646
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4481
  Illegal XbaI site found at 4646
  Illegal SpeI site found at 2347
  Illegal PstI site found at 2361
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3520
  Illegal BsaI.rc site found at 4505