Part:BBa_I732953

Ptemplate1 + RBS.ARL2A0301

Genotype	Character	Name
Top10/pT-TERM	So not express any repressors	CR00
Top10/pT-ARL4A0604	Constitutively express ARL4A0604	CR01
Top10/pT-ARL2A0203	Constitutively express ARL2A0203	CR10
Top10/pTet-ARL4A0604-ARL2A203	Constitutively express ARL4A0604 and ARL2A0203	CR11

• RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.

The original part is now BBa_I732952-I732812.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 1105
  Illegal EcoRI site found at 3240
  Illegal XbaI site found at 3405
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3240
  Illegal SpeI site found at 1106
  Illegal PstI site found at 1120
  Illegal NotI site found at 1113
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3240
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 1106
  Illegal EcoRI site found at 3240
  Illegal XbaI site found at 3405
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3240
  Illegal XbaI site found at 3405
  Illegal SpeI site found at 1106
  Illegal PstI site found at 1120
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2279
  Illegal BsaI.rc site found at 3264