Intermediate
Part:BBa_I732834
Designed by: Zhan Jian Group: iGEM07_USTC (2007-10-14)
ARL2A0404 (RBS+, TERM+)
The artificial repressor comes from the results by [http://2007.igem.org/USTC/Repressor_Evolution_on_Plates Repressor Evolution on Plates]. The natural lactose repressor protein has been redesigned at Position 17, 18 of a.a. sequence, which is the composition of amino acid of recognition helix (Figure I). Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly.
Usage and Biology
- This artificial lac-repressor can bind to the specific promoters with high affinity while weakly bind to other promoters with relatively low affinity. The repression intensity is indicated in the Repression Matrix shown as below. How to acquire this repressor and how to obtain Repressor-Promoter pairs will been shown in [http://2007.igem.org/USTC/Repressor_Evolution_on_Plates This Page].
- This part contains the CDS of the repressor, a strong RBS and a terminator. The same repressor CDS without RBS can be found at BBa_I732114 as a Standard BioBrick part, and with RBS at BBa_I732814 as a Standard BioBrick part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |