Part:BBa_I732814

ARL2A0404 (RBS+, TERM-)

Figure I The recognition region of Lac repressor of which the amino acid residues we will try to modify. This figure comes from 'Roberto Kopke Salinas, et al. ChemBioChem 2005, 6, 1628 - 1637

The artificial repressor comes from the results by [http://2007.igem.org/USTC/Repressor_Evolution_on_Plates Repressor Evolution on Plates]. The natural lactose repressor protein has been redesigned at Position 17, 18 of a.a. sequence, which is the composition of amino acid of recognition helix (Figure I). Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly.

Usage and Biology

• This artificial lac-repressor can bind to the specific promoters with high affinity while weakly bind to other promoters with relatively low affinity. The repression intensity is indicated in the Repression Matrix shown as below. How to acquire this repressor and how to obtain Repressor-Promoter pairs will been shown in [http://2007.igem.org/USTC/Repressor_Evolution_on_Plates This Page].

• This part contains the CDS of the repressor and a strong RBS. The same repressor CDS without RBS can be found at BBa_I732114 as a Standard BioBrick part, and with both RBS and terminator at BBa_I732834 as a Standard BioBrick part.

Figure II Repression Metrix(RM):The repression matrix reveals the binding affinity of different repressor candidates with various specific promoters by different colors. The deeper the red will be, the higher the repression will appear. While the lighter the blue is the weaker the repression will show.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]