Measurement

Part:BBa_I732701

Designed by: Zhan Jian   Group: iGEM07_USTC   (2007-10-14)


P_NOT_D001O11 + Double Reporters

P_NOT_D001O11 inserted into the promoter adapter of DPv2F (pSB3K5-I732096) using XbaI and BamHI. This plasmid is constructed for repressor screening and repression assay. The activity of P_NOT_D001O11 can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 18
    Illegal BsaI.rc site found at 1054


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