Part:BBa_I732100

LacI

Figure 1 Solution structure of a dimer of Lac repressor DNA-binding domain complexed to its natural operator O1 ([http://www.rcsb.org/pdb/explore.do?structureId=1L1M From RCSB])

The sequence produce LacI repressor protein.Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly.

Usage and Biology

The lac repressor is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. There are three distinct regions in the protein. The headpiece, a fragment contains approximately 51 amino acids from its N-terminal together with a helix-turn-helix motif, is the only region that serves to bind DNA. Figure I shows the NMR structure of the complex with two headpieces of the lac repressor and its nature operator.

(Reference: 1.Lewis, M. (2005), 'The lac repressor.', C R Biol 328 (6), 521--548. 2.Lehming, N.; Sartorius, J.; Oehler, S.; von Wilcken-Bergmann, B. & Müller-Hill, B. Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences. PNAS, 1988, 85, 7947-7951 3. Sartorius, J.; Lehming, N.; Kisters, B.; von Wilcken-Bergmann, B. & Müller-Hill, B. (1989), 'lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchanges.', EMBO J 8(4), 1265--1270. )

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]