Composite

Part:BBa_I732014

Designed by: Zhan Jian   Group: iGEM07_USTC   (2007-07-09)

[aTc] -> PoPS

[aTc]->PoPs converter. When [http://openwetware.org/wiki/ATc aTc (anhydrotetracycline)] is present, the repression of Ptet (Part:BBa_R0040) by constitutively expressed tetR(Part:BBa_C0040)(regulated by constitutive promoter PlacIQ(Part:BBa_I14032)) is relieved. Thus the downstream sequence of Ptet could be transcribed. The tetR protein we use here is LVA-tagged, to reduce its half life for better fast kinetics. However, the data from our measurement shows that this converter is not fit for this project and is abandoned at last.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None