Coding

Part:BBa_I732003

Designed by: Jian ZHAN   Group: iGEM07_USTC   (2007-06-16)


E.coli gyrA gene (quinolone-resistant)

Quinolone-resistant mutations of the E.coli DNA gyrase subunit A (EC 5.99.1.3). the four spontaneous gyrA mutants show variant levels of resistance to quinolones.

Data kindly reviewed (07-SEP-1988) by YOSHIDA H.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1593
    Illegal BglII site found at 2538
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 27
    Illegal AgeI site found at 703
    Illegal AgeI site found at 760
    Illegal AgeI site found at 1399
    Illegal AgeI site found at 2155
    Illegal AgeI site found at 2523
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 43
    Illegal SapI.rc site found at 51
    Illegal SapI.rc site found at 1510


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Parameters
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