Signalling

Part:BBa_I731014

Designed by: George Xu   Group: iGEM07_Harvard   (2007-10-14)


The luxR based receiver, F2620 (formerly I13270), controls the production of mCherry

This part is similar to Bba_T9002 except the reporter is mCherry (Bba_J06702) rather than GFP (Bba_E0240). Transcription of the luxR (receiver protein) gene is regulated by a Ptet promoter. In the presence of high concentrations of OHHL (3OC6HSL), the luxR protein binds to OHHL and upregulated the luxpR promoter, causing transcription of the mCherry reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


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Categories
Parameters
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