Signalling

Part:BBa_I731013

Designed by: Stephanie Lo   Group: iGEM07_Harvard   (2007-10-14)


luxpR-regulated luxR + luxpR-regulated GFP

Quorum receiver with two separate luxpR promoters. In presence of OHHL, these promoters are upregulated, enforcing the positive feedback response and causing a spike in GFP fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005
    Illegal BsaI.rc site found at 1733


[edit]
Categories
Parameters
None