Composite

Part:BBa_I730014

Designed by: Arnau Montagud Aquino   Group: iGEM07_Valencia   (2007-10-11)

pOmpRm-ptetR-LacI repressor-YFP

pOmpR-pTetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP(AAV+)-terminator

R0083.R0040.B0034.C0012.E0434

intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.

it has the osmolarity-sensing promoter for the OmpC gene, as the input of the device. this mutant is said to (and characterised as) be weaker than regular pOmpR

as an output we have thought of the fluorescence protein YFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1313
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None