Part:BBa_I730012

Comparator device

Not meant to be used as a single construction, but as a two-plasmid device.

BBa_I730002 and BBa_I730003 are the two halves of this device, if one of them bears an ampicillin resistance gene and the other a kanamicine one, you could be able of detect whatever you want (assembling the 'sensing' parts upstream of both constructs) and have a wished output (assembling the 'output' parts downstreams of both constructs).

As a proof of principle, for our iGEM 2007 project we have designed a comparator that has pOmpR and pOmpRm as inputs and fluorescence outputs. But we are aware of the huge modularity and potential of this device.

Both halves have been fully sequenced!!

more info in [http://2007.igem.org/Valencia/Results our wiki]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2001
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]