Composite

Part:BBa_I730011

Designed by: Arnau Montagud Aquino   Group: iGEM07_Valencia   (2007-10-11)

pOmpR-ptetR-LacI repressor-YFP

pOmpR-pTetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP(AAV+)-terminator

R0082.R0040.B0034.C0012.E0434

intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.

it has the osmolarity-sensing promoter for the OmpC gene, as the input of the device.

as an output we have thought of the fluorescence protein YFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None