Composite

Part:BBa_I730010

Designed by: Arnau Montagud Aquino   Group: iGEM07_Valencia   (2007-10-11)

pOmpR-pLacI-TetR repressor-CFP

pOmpR-ptetR-strong RBS-LacI repressor(LVA+)-strong RBS-CFP(LVA+)-terminator

R0082.R0011.B0034.C0040.E0422

intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.

it has the osmolarity-sensing promoter for the OmpC gene, as the input of the device.

as an output we have thought of the fluorescence protein CFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None