Composite
Part:BBa_I730006
Designed by: Arnau Montagud Aquino Group: iGEM07_Valencia (2007-10-11)
pOmpR-pLacI-TetR repressor
pOmpR-ptetR-strong RBS-LacI repressor(LVA+)
R0082.R0011.B0034.C0040
intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.
it has the osmolarity-sensing promoter for the OmpC gene, as the input of the device.
more info at [http://2007.igem.org/Valencia/Lab_Work our wiki].
we have ligated this parts, but looks like there are some issues on its digestion behaviour... it yields an unwanted 400pb band.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |