Composite

Part:BBa_I728978

Designed by: Bernice Huang   Group: iGEM07_MIT   (2007-10-23)

Promoter+RBS+CPX(Circularly-Permuted OmpX for Surface Display of Polypeptides)

When transformed into escherichia coli, this part will result in the display of a polystyrene-binding peptide on the N-terminus of the second extracellular loop of circularly-permutated OmpX (CPX) that will increase e. coli's binding affinity to plastic. The peptide displayed may be switched with another using the restriction sites BgII and SalI flanking the polystyrene-binding peptide sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1172
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


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