Coding

Part:BBa_I728951

Designed by: aditya kohli   Group: iGEM07_MIT   (2008-04-30)


Human Matrix Metalloproteinase 1 (Mmp1)

This is the gene for the Human Matrix Metallopeptidase 1 (interstitial collagenase). Proteins in the metalloproteinase family are involved in breaking down the extracellular matrix in physiological processes. This gene encodes for an active interstitial collagenase that breaks down interstitial type I, II, and III collagen. For our project, this gene is intended to be expressed in E.Coli for the purposes of breaking down formed scar tissue in the heart. The collagenase secretion will be regulated to prevent over-secretion and damage to adjacent cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 723
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 53
    Illegal SpeI site found at 724
    Illegal PstI site found at 738
    Illegal NotI site found at 7
    Illegal NotI site found at 731
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 533
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 724
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 724
    Illegal PstI site found at 738
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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