Coding

Part:BBa_I728950

Designed by: aditya kohli   Group: iGEM07_MIT   (2008-04-29)


Periostin Coding Sequence

Coding sequence for periostin, a protein which is over-expressed in damaged myocardial tissues and cardiac development. We are hoping to clone this protein into E. coli and use it to promote generation of healthy heart tissue after cardiac arrest.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2532
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2533
    Illegal PstI site found at 2547
    Illegal NotI site found at 7
    Illegal NotI site found at 2540
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 100
    Illegal BamHI site found at 408
    Illegal BamHI site found at 2493
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2533
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2533
    Illegal PstI site found at 2547
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1692


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Categories
Parameters
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